Tissueruptor ii handbook

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Verified Bots that are allowed around the Internet because they help provide services we use day to day? Requests from malicious bots can pose as legitimate traffic. Occasionally, you may see this page while the site ensures that the connection is secure. Detect TB infection with confidence. Looking for a quick way to design experiments? A convenient tool to build experimental workflows and find products to match your needs. You can accept or revoke the cookies used on this website at any time with the selection below or by adjusting your cookie settings. The TissueRuptor II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample. The TissueRuptor II has all the same features and functionality as the old TissueRuptor. QIAGEN has tested use of the TissueRuptor II in combination with proven QIAGEN sample preparation kits. Successful purification of phosphoproteins. C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. High performance results in PCR. K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. RNA Mini Kit and analyzed by agarose gel electrophoresis. Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs. Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II. The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material. After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process. The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits. Efficient disruption and homogenization of animal tissues. Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor. Rapid, effective disruption of a range of sample types. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies. However, shorter DNA fragments are easier to elute, so DNA yields will be higher. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower. Tissue Kit or the QIAamp DNA Mini Kit? Efficient DNA isolation requires thorough sample disruption and digestion. K after several hours of digestion. Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent? Custom LNA Oligos for applications where no standard product is available. Choose from a wide range of labels and modifications. Multiplex amplification for human ID, with CODIS and European standard markers. NGS interpretation and reporting. Explore tailored OEM solutions for life science and molecular diagnostics. Streamline target exploration, product configuration, data analysis and experiment planning. Get answers to questions about QIAGEN, our products or your order. Quality and safety documentation for your products. Do you know someone who is starting a new lab? Find protocols, product documentation, software, FAQs, videos, webinars, and more. Learn about who we are and what we do to make improvements in life possible. Find all resources covering diagnostic testing, research solutions and latest news in one place. Explore our digital magazine full of customer stories and videos on scientific breakthroughs and healthcare advances. Read our equity story, financial disclosures and how we create shareholder value. Read up on our latest press releases or peruse our various media archives. If you have questions ab filexlib.
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